ssr_primer
Primer Details for TRINITY_DN11108_c4_g3_i5 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATCGCACTTGGTTGTTGC | GTTGCAGATTTCGGGATCGC | Start: 897 |
| Tm | 59.969 | 59.972 | End: 1111 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | GCAGAAGCTGAACCACCTCT | GCAACAACCAAGTGCGATGT | Start: 640 |
| Tm | 59.964 | 59.969 | End: 916 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GGCACAGGTGAAGAAGACGA | TGTTCCACCGGCGTATTTCA | Start: 1655 |
| Tm | 59.966 | 59.965 | End: 1781 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | GACGGCACAGGTGAAGAAGA | TGTTCCACCGGCGTATTTCA | Start: 1652 |
| Tm | 59.966 | 59.965 | End: 1781 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | GATCCACCGTGACCCACAAT | TCTTCTTCACCTGTGCCGTC | Start: 1500 |
| Tm | 60.035 | 59.966 | End: 1671 |
| Size | 20 | 20 | 172 |
(AG)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATCGCACTTGGTTGTTGC | GTTGCAGATTTCGGGATCGC | Start: 897 |
| Tm | 59.969 | 59.972 | End: 1111 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | GCAGAAGCTGAACCACCTCT | GCAACAACCAAGTGCGATGT | Start: 640 |
| Tm | 59.964 | 59.969 | End: 916 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GGCACAGGTGAAGAAGACGA | TGTTCCACCGGCGTATTTCA | Start: 1655 |
| Tm | 59.966 | 59.965 | End: 1781 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | GACGGCACAGGTGAAGAAGA | TGTTCCACCGGCGTATTTCA | Start: 1652 |
| Tm | 59.966 | 59.965 | End: 1781 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | GATCCACCGTGACCCACAAT | TCTTCTTCACCTGTGCCGTC | Start: 1500 |
| Tm | 60.035 | 59.966 | End: 1671 |
| Size | 20 | 20 | 172 |
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