ssr_primer
Primer Details for TRINITY_DN11108_c4_g10_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGGGTGTATTTCTGTTGC | AACAAACCCTCGATCCACCC | Start: 295 |
| Tm | 59.83 | 59.962 | End: 515 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | ATTGTTGGTTGTCGGTCGGT | AACAAACCCTCGATCCACCC | Start: 270 |
| Tm | 60.179 | 59.962 | End: 515 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | TTCTGCCCTCCCTTCCAAAC | ACCGACCGACAACCAACAAT | Start: 10 |
| Tm | 59.888 | 60.179 | End: 289 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | CGCTTCTTGCCATTGGTCAG | GCAACAGAAATACACCCCGC | Start: 86 |
| Tm | 59.83 | 59.83 | End: 314 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
|---|
| Sequence | CGCTTCTTGCCATTGGTCAG | ACCGACCGACAACCAACAAT | Start: 86 |
| Tm | 59.83 | 60.179 | End: 289 |
| Size | 20 | 20 | 204 |
(T)54: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGGGTGTATTTCTGTTGC | AACAAACCCTCGATCCACCC | Start: 295 |
| Tm | 59.83 | 59.962 | End: 515 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | ATTGTTGGTTGTCGGTCGGT | AACAAACCCTCGATCCACCC | Start: 270 |
| Tm | 60.179 | 59.962 | End: 515 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | TTCTGCCCTCCCTTCCAAAC | ACCGACCGACAACCAACAAT | Start: 10 |
| Tm | 59.888 | 60.179 | End: 289 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | CGCTTCTTGCCATTGGTCAG | GCAACAGAAATACACCCCGC | Start: 86 |
| Tm | 59.83 | 59.83 | End: 314 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
|---|
| Sequence | CGCTTCTTGCCATTGGTCAG | ACCGACCGACAACCAACAAT | Start: 86 |
| Tm | 59.83 | 60.179 | End: 289 |
| Size | 20 | 20 | 204 |
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