ssr_primer
Primer Details for TRINITY_DN11080_c2_g6_i5 |
|---|
| (TC)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCGATGATCGATGCCCAG | TCAACTGCGTGGACTCTCAC | Start: 730 |
| Tm | 59.966 | 59.968 | End: 928 |
| Size | 20 | 20 | 199 |
| Primer 2 details |
|---|
| Sequence | CTTCCGATGATCGATGCCCA | TCAACTGCGTGGACTCTCAC | Start: 729 |
| Tm | 59.966 | 59.968 | End: 928 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | TCGCTCGATCAATCTGGAGC | TCTTCAGTTCTTGGCGGACC | Start: 3225 |
| Tm | 59.969 | 59.965 | End: 3333 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | TTCCGATGATCGATGCCCAG | CTGCGTGGACTCTCACATGT | Start: 730 |
| Tm | 59.966 | 60.038 | End: 924 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
|---|
| Sequence | TTCCGATGATCGATGCCCAG | ACTGCGTGGACTCTCACATG | Start: 730 |
| Tm | 59.966 | 60.038 | End: 925 |
| Size | 20 | 20 | 196 |
(T)10(TTC)10*: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCGATGATCGATGCCCAG | TCAACTGCGTGGACTCTCAC | Start: 730 |
| Tm | 59.966 | 59.968 | End: 928 |
| Size | 20 | 20 | 199 |
| Primer 2 details |
|---|
| Sequence | CTTCCGATGATCGATGCCCA | TCAACTGCGTGGACTCTCAC | Start: 729 |
| Tm | 59.966 | 59.968 | End: 928 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | TCGCTCGATCAATCTGGAGC | TCTTCAGTTCTTGGCGGACC | Start: 3225 |
| Tm | 59.969 | 59.965 | End: 3333 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | TTCCGATGATCGATGCCCAG | CTGCGTGGACTCTCACATGT | Start: 730 |
| Tm | 59.966 | 60.038 | End: 924 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
|---|
| Sequence | TTCCGATGATCGATGCCCAG | ACTGCGTGGACTCTCACATG | Start: 730 |
| Tm | 59.966 | 60.038 | End: 925 |
| Size | 20 | 20 | 196 |
(T)11aaataaaaaaagag(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCGATGATCGATGCCCAG | TCAACTGCGTGGACTCTCAC | Start: 730 |
| Tm | 59.966 | 59.968 | End: 928 |
| Size | 20 | 20 | 199 |
| Primer 2 details |
|---|
| Sequence | CTTCCGATGATCGATGCCCA | TCAACTGCGTGGACTCTCAC | Start: 729 |
| Tm | 59.966 | 59.968 | End: 928 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | TCGCTCGATCAATCTGGAGC | TCTTCAGTTCTTGGCGGACC | Start: 3225 |
| Tm | 59.969 | 59.965 | End: 3333 |
| Size | 20 | 20 | 109 |
| Primer 4 details |
|---|
| Sequence | TTCCGATGATCGATGCCCAG | CTGCGTGGACTCTCACATGT | Start: 730 |
| Tm | 59.966 | 60.038 | End: 924 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
|---|
| Sequence | TTCCGATGATCGATGCCCAG | ACTGCGTGGACTCTCACATG | Start: 730 |
| Tm | 59.966 | 60.038 | End: 925 |
| Size | 20 | 20 | 196 |
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