ssr_primer
Primer Details for TRINITY_DN11069_c3_g1_i1 |
|---|
| (TGG)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCAGAAACCACCAGCTTTG | CAACGTGCGCCAATTCTTCA | Start: 96 |
| Tm | 59.967 | 60.041 | End: 311 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
|---|
| Sequence | GCCACGGCCAGAAGTCTTAT | TATCTGTCCCAAACCACGGC | Start: 813 |
| Tm | 60.108 | 60.036 | End: 1066 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | TTTGATTCTGCCACCGAGCT | GAGGCCCAAAGTCGCTATGT | Start: 586 |
| Tm | 59.963 | 60.108 | End: 685 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | TTTGATTCTGCCACCGAGCT | ATAAGACTTCTGGCCGTGGC | Start: 586 |
| Tm | 59.963 | 60.108 | End: 832 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
|---|
| Sequence | TGAATCGAATCCCACACGCA | ATAAGACTTCTGGCCGTGGC | Start: 644 |
| Tm | 60.037 | 60.108 | End: 832 |
| Size | 20 | 20 | 189 |
(CCG)5ccacggccagaagtcttatgcttaggatgcaacaaatcccaacagaaattagagacataagaaccagcggaacggcggtggtgattacgtgca(GCG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCAGAAACCACCAGCTTTG | CAACGTGCGCCAATTCTTCA | Start: 96 |
| Tm | 59.967 | 60.041 | End: 311 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
|---|
| Sequence | GCCACGGCCAGAAGTCTTAT | TATCTGTCCCAAACCACGGC | Start: 813 |
| Tm | 60.108 | 60.036 | End: 1066 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | TTTGATTCTGCCACCGAGCT | GAGGCCCAAAGTCGCTATGT | Start: 586 |
| Tm | 59.963 | 60.108 | End: 685 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | TTTGATTCTGCCACCGAGCT | ATAAGACTTCTGGCCGTGGC | Start: 586 |
| Tm | 59.963 | 60.108 | End: 832 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
|---|
| Sequence | TGAATCGAATCCCACACGCA | ATAAGACTTCTGGCCGTGGC | Start: 644 |
| Tm | 60.037 | 60.108 | End: 832 |
| Size | 20 | 20 | 189 |
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