ssr_primer
Primer Details for TRINITY_DN11049_c1_g1_i1 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGCAAAGCAGGGACATCC | ACGGCAAAGTCGTACTCGTT | Start: 4233 |
| Tm | 60.035 | 59.969 | End: 4347 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | AACGAGTACGACTTTGCCGT | ACGGATACTCCTCTGGACCC | Start: 4328 |
| Tm | 59.969 | 60.106 | End: 4518 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
|---|
| Sequence | AGAGCAAAGCAGGGACATCC | TCTACGCCAAAGCAAACCCT | Start: 4233 |
| Tm | 60.035 | 59.89 | End: 4389 |
| Size | 20 | 20 | 157 |
| Primer 4 details |
|---|
| Sequence | AGGGTTTGCTTTGGCGTAGA | CTCCACTGGTTCCCCACATC | Start: 4370 |
| Tm | 59.89 | 60.035 | End: 4635 |
| Size | 20 | 20 | 266 |
| Primer 5 details |
|---|
| Sequence | TGCCGTCATGAATTCCACCA | TGCAGGTTCAGTTGACACGT | Start: 2645 |
| Tm | 59.963 | 60.108 | End: 2815 |
| Size | 20 | 20 | 171 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGCAAAGCAGGGACATCC | ACGGCAAAGTCGTACTCGTT | Start: 4233 |
| Tm | 60.035 | 59.969 | End: 4347 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | AACGAGTACGACTTTGCCGT | ACGGATACTCCTCTGGACCC | Start: 4328 |
| Tm | 59.969 | 60.106 | End: 4518 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
|---|
| Sequence | AGAGCAAAGCAGGGACATCC | TCTACGCCAAAGCAAACCCT | Start: 4233 |
| Tm | 60.035 | 59.89 | End: 4389 |
| Size | 20 | 20 | 157 |
| Primer 4 details |
|---|
| Sequence | AGGGTTTGCTTTGGCGTAGA | CTCCACTGGTTCCCCACATC | Start: 4370 |
| Tm | 59.89 | 60.035 | End: 4635 |
| Size | 20 | 20 | 266 |
| Primer 5 details |
|---|
| Sequence | TGCCGTCATGAATTCCACCA | TGCAGGTTCAGTTGACACGT | Start: 2645 |
| Tm | 59.963 | 60.108 | End: 2815 |
| Size | 20 | 20 | 171 |
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