ssr_primer
Primer Details for TRINITY_DN10999_c2_g1_i4 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCTTACGCGTTGAATCGG | GAGAAGACCAAGTCCACCGG | Start: 4 |
| Tm | 59.975 | 60.037 | End: 274 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | CTGCTTACGCGTTGAATCGG | GGAGAAGACCAAGTCCACCG | Start: 4 |
| Tm | 59.975 | 60.037 | End: 275 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | CTGCTTACGCGTTGAATCGG | CGGAGAAGACCAAGTCCACC | Start: 4 |
| Tm | 59.975 | 60.037 | End: 276 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | CGATCTCTCCATCACCGACG | TCCAGCATCGGCAAAGTCAT | Start: 3612 |
| Tm | 60.041 | 60.035 | End: 3783 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | GGGAGAAGTGGAACAGTCGG | CGTCGGTGATGGAGAGATCG | Start: 3448 |
| Tm | 60.037 | 60.041 | End: 3631 |
| Size | 20 | 20 | 184 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCTTACGCGTTGAATCGG | GAGAAGACCAAGTCCACCGG | Start: 4 |
| Tm | 59.975 | 60.037 | End: 274 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | CTGCTTACGCGTTGAATCGG | GGAGAAGACCAAGTCCACCG | Start: 4 |
| Tm | 59.975 | 60.037 | End: 275 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | CTGCTTACGCGTTGAATCGG | CGGAGAAGACCAAGTCCACC | Start: 4 |
| Tm | 59.975 | 60.037 | End: 276 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | CGATCTCTCCATCACCGACG | TCCAGCATCGGCAAAGTCAT | Start: 3612 |
| Tm | 60.041 | 60.035 | End: 3783 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | GGGAGAAGTGGAACAGTCGG | CGTCGGTGATGGAGAGATCG | Start: 3448 |
| Tm | 60.037 | 60.041 | End: 3631 |
| Size | 20 | 20 | 184 |
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