ssr_primer
Primer Details for TRINITY_DN10981_c3_g7_i4 |
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| (T)28gtacccaaactattgcaatgaagggttgacatatgcattcaacctttc(AAT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCACGGTCGGTTTAGAACA | GGCTCTGACATGGGAAGCTT | Start: 1038 |
| Tm | 59.966 | 60.035 | End: 1176 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
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| Sequence | CCCACGGTCGGTTTAGAACA | TGGTTAGCAGAACGAGCAGG | Start: 1038 |
| Tm | 59.966 | 60.037 | End: 1305 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
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| Sequence | AAGCTTCCCATGTCAGAGCC | TGGTTAGCAGAACGAGCAGG | Start: 1157 |
| Tm | 60.035 | 60.037 | End: 1305 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | CCCACGGTCGGTTTAGAACA | TACCTGGCTCTGACATGGGA | Start: 1038 |
| Tm | 59.966 | 59.957 | End: 1181 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TCCCATGTCAGAGCCAGGTA | TGGTTAGCAGAACGAGCAGG | Start: 1162 |
| Tm | 59.957 | 60.037 | End: 1305 |
| Size | 20 | 20 | 144 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCACGGTCGGTTTAGAACA | GGCTCTGACATGGGAAGCTT | Start: 1038 |
| Tm | 59.966 | 60.035 | End: 1176 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
|---|
| Sequence | CCCACGGTCGGTTTAGAACA | TGGTTAGCAGAACGAGCAGG | Start: 1038 |
| Tm | 59.966 | 60.037 | End: 1305 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | AAGCTTCCCATGTCAGAGCC | TGGTTAGCAGAACGAGCAGG | Start: 1157 |
| Tm | 60.035 | 60.037 | End: 1305 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
|---|
| Sequence | CCCACGGTCGGTTTAGAACA | TACCTGGCTCTGACATGGGA | Start: 1038 |
| Tm | 59.966 | 59.957 | End: 1181 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TCCCATGTCAGAGCCAGGTA | TGGTTAGCAGAACGAGCAGG | Start: 1162 |
| Tm | 59.957 | 60.037 | End: 1305 |
| Size | 20 | 20 | 144 |
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