ssr_primer
Primer Details for TRINITY_DN10903_c4_g1_i3 |
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| (AT)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGATGCGGCCAAAGAATCAG | CCCAGATGACGAGCTGTTGT | Start: 94 |
| Tm | 59.971 | 60.037 | End: 368 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
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| Sequence | TCGGGAGAGGAGCATCAAGA | TGCTCTGGCAGAGTTCAAGG | Start: 3648 |
| Tm | 60.033 | 59.964 | End: 3892 |
| Size | 20 | 20 | 245 |
| Primer 3 details |
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| Sequence | CCTTGAACTCTGCCAGAGCA | ATGCGAGAGTTTCAAGGCGA | Start: 3873 |
| Tm | 59.964 | 60.037 | End: 4084 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
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| Sequence | TCGGGAGAGGAGCATCAAGA | GCATCTGCGCCTGCTTATTC | Start: 3648 |
| Tm | 60.033 | 60.041 | End: 3759 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
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| Sequence | GAATAAGCAGGCGCAGATGC | TGCTCTGGCAGAGTTCAAGG | Start: 3740 |
| Tm | 60.041 | 59.964 | End: 3892 |
| Size | 20 | 20 | 153 |
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