ssr_primer
Primer Details for TRINITY_DN10899_c3_g1_i16 |
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| (A)10cttttacaatatacaacacaattttaaaatggagtaggaagactttgacgaattttgtattttgttatttatgacatcgtccattttttccc(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATGTGGCTAGGGTTCCCT | TCTCAACGCAGGTTCCTGAC | Start: 6518 |
| Tm | 59.955 | 59.966 | End: 6649 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | AGATGTGGCTAGGGTTCCCT | CTCAACGCAGGTTCCTGACT | Start: 6518 |
| Tm | 59.955 | 59.966 | End: 6648 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | AGATGTGGCTAGGGTTCCCT | CCAAGGATCTCAACGCAGGT | Start: 6518 |
| Tm | 59.955 | 60.036 | End: 6656 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | AGGACACGAGTTTCACCACC | CGGAATCGCTGCCAAATCTG | Start: 7131 |
| Tm | 59.894 | 59.971 | End: 7253 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
|---|
| Sequence | GTCAGGAACCTGCGTTGAGA | TCCGATGAGGACCTAGCGAA | Start: 6630 |
| Tm | 59.966 | 60.107 | End: 6842 |
| Size | 20 | 20 | 213 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATGTGGCTAGGGTTCCCT | TCTCAACGCAGGTTCCTGAC | Start: 6518 |
| Tm | 59.955 | 59.966 | End: 6649 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | AGATGTGGCTAGGGTTCCCT | CTCAACGCAGGTTCCTGACT | Start: 6518 |
| Tm | 59.955 | 59.966 | End: 6648 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | AGATGTGGCTAGGGTTCCCT | CCAAGGATCTCAACGCAGGT | Start: 6518 |
| Tm | 59.955 | 60.036 | End: 6656 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | AGGACACGAGTTTCACCACC | CGGAATCGCTGCCAAATCTG | Start: 7131 |
| Tm | 59.894 | 59.971 | End: 7253 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
|---|
| Sequence | GTCAGGAACCTGCGTTGAGA | TCCGATGAGGACCTAGCGAA | Start: 6630 |
| Tm | 59.966 | 60.107 | End: 6842 |
| Size | 20 | 20 | 213 |
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