ssr_primer
Primer Details for TRINITY_DN10888_c4_g1_i3 |
|---|
| (TCT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCCTGAAGAACCTGCAGCC | AAAGGTGGCTGTACATCCGG | Start: 772 |
| Tm | 60.035 | 60.035 | End: 991 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | TTCCCTTCCCTTGTTCTCGC | GGCTGCAGGTTCTTCAGGAT | Start: 680 |
| Tm | 59.964 | 60.035 | End: 791 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | CGCTTCCCTTCCCTTGTTCT | GGCTGCAGGTTCTTCAGGAT | Start: 677 |
| Tm | 59.963 | 60.035 | End: 791 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
|---|
| Sequence | GGCAGATGGGTTCTCACACA | AAAGGTGGCTGTACATCCGG | Start: 746 |
| Tm | 59.963 | 60.035 | End: 991 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | TGGCAGATGGGTTCTCACAC | AAAGGTGGCTGTACATCCGG | Start: 745 |
| Tm | 59.963 | 60.035 | End: 991 |
| Size | 20 | 20 | 247 |
(A)16tctccaagaaagtggaaaacaagagaggagggaaattaaaaaagaaaatagaacacacacac(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCCTGAAGAACCTGCAGCC | AAAGGTGGCTGTACATCCGG | Start: 772 |
| Tm | 60.035 | 60.035 | End: 991 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | TTCCCTTCCCTTGTTCTCGC | GGCTGCAGGTTCTTCAGGAT | Start: 680 |
| Tm | 59.964 | 60.035 | End: 791 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | CGCTTCCCTTCCCTTGTTCT | GGCTGCAGGTTCTTCAGGAT | Start: 677 |
| Tm | 59.963 | 60.035 | End: 791 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
|---|
| Sequence | GGCAGATGGGTTCTCACACA | AAAGGTGGCTGTACATCCGG | Start: 746 |
| Tm | 59.963 | 60.035 | End: 991 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | TGGCAGATGGGTTCTCACAC | AAAGGTGGCTGTACATCCGG | Start: 745 |
| Tm | 59.963 | 60.035 | End: 991 |
| Size | 20 | 20 | 247 |
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