ssr_primer
Primer Details for TRINITY_DN10696_c0_g1_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTTTTGCTGCAGGCGGAAG | GACGGAAGCTCTCGAAACCA | Start: 1211 |
| Tm | 60.038 | 60.039 | End: 1366 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | GATTTTTGCTGCAGGCGGAA | GACGGAAGCTCTCGAAACCA | Start: 1210 |
| Tm | 60.038 | 60.039 | End: 1366 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | TCAAGGACGAGTTTGCACGA | AACCATGGACGTCGACGAAA | Start: 2058 |
| Tm | 59.897 | 59.968 | End: 2186 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
|---|
| Sequence | CCCAGTGCCAGGTGAAGATT | TCCTTCCAGGTTCCAAGGGA | Start: 33 |
| Tm | 59.961 | 60.104 | End: 177 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | GTGGGGTCCCGACATCATTT | TCAGCTGAGACCCCAAACAC | Start: 940 |
| Tm | 60.034 | 59.891 | End: 1039 |
| Size | 20 | 20 | 100 |
(T)10gtatcatagcacgacatttagcgagaggctttgacccg(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTTTTGCTGCAGGCGGAAG | GACGGAAGCTCTCGAAACCA | Start: 1211 |
| Tm | 60.038 | 60.039 | End: 1366 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | GATTTTTGCTGCAGGCGGAA | GACGGAAGCTCTCGAAACCA | Start: 1210 |
| Tm | 60.038 | 60.039 | End: 1366 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | TCAAGGACGAGTTTGCACGA | AACCATGGACGTCGACGAAA | Start: 2058 |
| Tm | 59.897 | 59.968 | End: 2186 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
|---|
| Sequence | CCCAGTGCCAGGTGAAGATT | TCCTTCCAGGTTCCAAGGGA | Start: 33 |
| Tm | 59.961 | 60.104 | End: 177 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | GTGGGGTCCCGACATCATTT | TCAGCTGAGACCCCAAACAC | Start: 940 |
| Tm | 60.034 | 59.891 | End: 1039 |
| Size | 20 | 20 | 100 |
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