Primer Details for TRINITY_DN10639_c5_g1_i2 |
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| (A)10tttaatatgatgg(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGTACCTTAATTTATCTTGGGGA | TCGGAGCCATAAATCCAACTT | Start: 151 |
| Tm | 59.571 | 57.355 | End: 263 |
| Size | 25 | 21 | 113 |
| Primer 2 details |
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| Sequence | TCTTGGGGATGCATAAGAATTTGG | AAATCGGAGCCATAAATCCAACT | Start: 167 |
| Tm | 59.349 | 58.463 | End: 266 |
| Size | 24 | 23 | 100 |
| Primer 3 details |
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| Sequence | ACCGTACCTTAATTTATCTTGGGGA | ATCGGAGCCATAAATCCAACT | Start: 151 |
| Tm | 59.571 | 57.135 | End: 264 |
| Size | 25 | 21 | 114 |
| Primer 4 details |
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| Sequence | ACCGTACCTTAATTTATCTTGGGG | TCGGAGCCATAAATCCAACTT | Start: 151 |
| Tm | 58.312 | 57.355 | End: 263 |
| Size | 24 | 21 | 113 |
| Primer 5 details |
|---|
| Sequence | ACCGTACCTTAATTTATCTTGGGGA | TCGGAGCCATAAATCCAACTTT | Start: 151 |
| Tm | 59.571 | 58.037 | End: 263 |
| Size | 25 | 22 | 113 |
(TA)8tgtttttaaaaatgttgaaatttttaaatattttaaatatctcaataaaagttaaacttgagatttatcc(A)11gaaataatttaaaataaaataagataattggattatcttttctt(TA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGTACCTTAATTTATCTTGGGGA | TCGGAGCCATAAATCCAACTT | Start: 151 |
| Tm | 59.571 | 57.355 | End: 263 |
| Size | 25 | 21 | 113 |
| Primer 2 details |
|---|
| Sequence | TCTTGGGGATGCATAAGAATTTGG | AAATCGGAGCCATAAATCCAACT | Start: 167 |
| Tm | 59.349 | 58.463 | End: 266 |
| Size | 24 | 23 | 100 |
| Primer 3 details |
|---|
| Sequence | ACCGTACCTTAATTTATCTTGGGGA | ATCGGAGCCATAAATCCAACT | Start: 151 |
| Tm | 59.571 | 57.135 | End: 264 |
| Size | 25 | 21 | 114 |
| Primer 4 details |
|---|
| Sequence | ACCGTACCTTAATTTATCTTGGGG | TCGGAGCCATAAATCCAACTT | Start: 151 |
| Tm | 58.312 | 57.355 | End: 263 |
| Size | 24 | 21 | 113 |
| Primer 5 details |
|---|
| Sequence | ACCGTACCTTAATTTATCTTGGGGA | TCGGAGCCATAAATCCAACTTT | Start: 151 |
| Tm | 59.571 | 58.037 | End: 263 |
| Size | 25 | 22 | 113 |
(T)16gtgtgttttttattctgttttgttttgttttttttgtttgttttgttttttgtgttgttttg(T)45: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGTACCTTAATTTATCTTGGGGA | TCGGAGCCATAAATCCAACTT | Start: 151 |
| Tm | 59.571 | 57.355 | End: 263 |
| Size | 25 | 21 | 113 |
| Primer 2 details |
|---|
| Sequence | TCTTGGGGATGCATAAGAATTTGG | AAATCGGAGCCATAAATCCAACT | Start: 167 |
| Tm | 59.349 | 58.463 | End: 266 |
| Size | 24 | 23 | 100 |
| Primer 3 details |
|---|
| Sequence | ACCGTACCTTAATTTATCTTGGGGA | ATCGGAGCCATAAATCCAACT | Start: 151 |
| Tm | 59.571 | 57.135 | End: 264 |
| Size | 25 | 21 | 114 |
| Primer 4 details |
|---|
| Sequence | ACCGTACCTTAATTTATCTTGGGG | TCGGAGCCATAAATCCAACTT | Start: 151 |
| Tm | 58.312 | 57.355 | End: 263 |
| Size | 24 | 21 | 113 |
| Primer 5 details |
|---|
| Sequence | ACCGTACCTTAATTTATCTTGGGGA | TCGGAGCCATAAATCCAACTTT | Start: 151 |
| Tm | 59.571 | 58.037 | End: 263 |
| Size | 25 | 22 | 113 |
