ssr_primer
Primer Details for TRINITY_DN10623_c0_g2_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATAGATCTTGGTGGGCAGCG | AGTTGGAAATTGTGGACGGA | Start: 394 |
| Tm | 59.893 | 57.35 | End: 531 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
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| Sequence | ATAGATCTTGGTGGGCAGCG | AAGTTGGAAATTGTGGACGGA | Start: 394 |
| Tm | 59.893 | 58.061 | End: 532 |
| Size | 20 | 21 | 139 |
| Primer 3 details |
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| Sequence | ATAGATCTTGGTGGGCAGCG | AGTTGGAAATTGTGGACGGAT | Start: 394 |
| Tm | 59.893 | 57.845 | End: 531 |
| Size | 20 | 21 | 138 |
| Primer 4 details |
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| Sequence | AGCGGTCAAAATCTCCGAGC | AGTTGGAAATTGTGGACGGA | Start: 410 |
| Tm | 60.741 | 57.35 | End: 531 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
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| Sequence | ATAGATCTTGGTGGGCAGCG | ACACAATGGAAGTGCAGTTTCT | Start: 394 |
| Tm | 59.893 | 58.709 | End: 611 |
| Size | 20 | 22 | 218 |
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