ssr_primer
Primer Details for TRINITY_DN10553_c0_g1_i2 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATGCCATCCAAGCAAGCT | TTGATGCTGGGATCGATCGG | Start: 790 |
| Tm | 60.033 | 59.966 | End: 1042 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TGCCATCCAAGCAAGCTACA | TTGATGCTGGGATCGATCGG | Start: 793 |
| Tm | 59.962 | 59.966 | End: 1042 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
|---|
| Sequence | TCGCCCAATAAGCATGTCGT | AGCTTGCTTGGATGGCATCT | Start: 532 |
| Tm | 60.108 | 60.033 | End: 809 |
| Size | 20 | 20 | 278 |
| Primer 4 details |
|---|
| Sequence | AGATGCCATCCAAGCAAGCT | GAGCCACCCTCATCAGACAC | Start: 790 |
| Tm | 60.033 | 60.108 | End: 938 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | AGATGCCATCCAAGCAAGCT | GTCAAGGAATTACGGCGGGA | Start: 790 |
| Tm | 60.033 | 60.108 | End: 956 |
| Size | 20 | 20 | 167 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATGCCATCCAAGCAAGCT | TTGATGCTGGGATCGATCGG | Start: 790 |
| Tm | 60.033 | 59.966 | End: 1042 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TGCCATCCAAGCAAGCTACA | TTGATGCTGGGATCGATCGG | Start: 793 |
| Tm | 59.962 | 59.966 | End: 1042 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
|---|
| Sequence | TCGCCCAATAAGCATGTCGT | AGCTTGCTTGGATGGCATCT | Start: 532 |
| Tm | 60.108 | 60.033 | End: 809 |
| Size | 20 | 20 | 278 |
| Primer 4 details |
|---|
| Sequence | AGATGCCATCCAAGCAAGCT | GAGCCACCCTCATCAGACAC | Start: 790 |
| Tm | 60.033 | 60.108 | End: 938 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | AGATGCCATCCAAGCAAGCT | GTCAAGGAATTACGGCGGGA | Start: 790 |
| Tm | 60.033 | 60.108 | End: 956 |
| Size | 20 | 20 | 167 |
(GA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATGCCATCCAAGCAAGCT | TTGATGCTGGGATCGATCGG | Start: 790 |
| Tm | 60.033 | 59.966 | End: 1042 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TGCCATCCAAGCAAGCTACA | TTGATGCTGGGATCGATCGG | Start: 793 |
| Tm | 59.962 | 59.966 | End: 1042 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
|---|
| Sequence | TCGCCCAATAAGCATGTCGT | AGCTTGCTTGGATGGCATCT | Start: 532 |
| Tm | 60.108 | 60.033 | End: 809 |
| Size | 20 | 20 | 278 |
| Primer 4 details |
|---|
| Sequence | AGATGCCATCCAAGCAAGCT | GAGCCACCCTCATCAGACAC | Start: 790 |
| Tm | 60.033 | 60.108 | End: 938 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | AGATGCCATCCAAGCAAGCT | GTCAAGGAATTACGGCGGGA | Start: 790 |
| Tm | 60.033 | 60.108 | End: 956 |
| Size | 20 | 20 | 167 |
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