ssr_primer
Primer Details for TRINITY_DN10546_c0_g1_i3 |
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| (GCT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCAGCAGGAATCCGAAGG | CGCCATTGATAGTTGCCACG | Start: 4009 |
| Tm | 60.035 | 59.972 | End: 4216 |
| Size | 20 | 20 | 208 |
| Primer 2 details |
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| Sequence | GTACTGGGAGGTGGTTTGGG | CAGCAGAAGAGGTCGAAGCA | Start: 921 |
| Tm | 59.962 | 60.038 | End: 1069 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
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| Sequence | TACTGGGAGGTGGTTTGGGA | CAGCAGAAGAGGTCGAAGCA | Start: 922 |
| Tm | 60.104 | 60.038 | End: 1069 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
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| Sequence | GTACTGGGAGGTGGTTTGGG | CCCAGCAGAAGAGGTCGAAG | Start: 921 |
| Tm | 59.962 | 60.108 | End: 1071 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
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| Sequence | CACCATCTCCCCACCCTTTC | TAGCTTCTGGGGACTGGGAA | Start: 147 |
| Tm | 60.034 | 59.882 | End: 273 |
| Size | 20 | 20 | 127 |
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