ssr_primer
Primer Details for TRINITY_DN10487_c0_g1_i7 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAGGGCCGAGAGACTGTA | CTTTGCAGGTTCCACCAAGC | Start: 846 |
| Tm | 59.961 | 59.967 | End: 1123 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
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| Sequence | CTTCTCTGTGGTGGCCTCAG | GAGCTTGGTCGGGTGGTAAA | Start: 296 |
| Tm | 60.037 | 59.964 | End: 545 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
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| Sequence | TGTAACAGGTGCTACTGGCG | TTTCACCCGAGTCCTCAAGC | Start: 1458 |
| Tm | 60.038 | 59.965 | End: 1627 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
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| Sequence | CGTGTTGAGTGCAATGGTGG | GAAGGGCACTGAAGCTCCAT | Start: 2843 |
| Tm | 60.04 | 60.035 | End: 2970 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
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| Sequence | CCGTGTTGAGTGCAATGGTG | GAAGGGCACTGAAGCTCCAT | Start: 2842 |
| Tm | 60.04 | 60.035 | End: 2970 |
| Size | 20 | 20 | 129 |
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