Primer Details for TRINITY_DN1047_c0_g1_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAAAATTAGCTAGATGCATGGA | CCCCGTCGCCATTACTACAA | Start: 180 |
| Tm | 58.551 | 59.824 | End: 291 |
| Size | 24 | 20 | 112 |
| Primer 2 details | |||
| Sequence | ACCAAAATTAGCTAGATGCATGG | CCCCGTCGCCATTACTACAA | Start: 180 |
| Tm | 57.22 | 59.824 | End: 291 |
| Size | 23 | 20 | 112 |
| Primer 3 details | |||
| Sequence | ACCAAAATTAGCTAGATGCATGGAA | CCCCGTCGCCATTACTACAA | Start: 180 |
| Tm | 59.107 | 59.824 | End: 291 |
| Size | 25 | 20 | 112 |
| Primer 4 details | |||
| Sequence | ACCAAAATTAGCTAGATGCATGGA | GATAGAGATCCTGCCCCGTC | Start: 180 |
| Tm | 58.551 | 59.108 | End: 304 |
| Size | 24 | 20 | 125 |
| Primer 5 details | |||
| Sequence | ACCAAAATTAGCTAGATGCATGGA | ATAGAGATCCTGCCCCGTCG | Start: 180 |
| Tm | 58.551 | 60.896 | End: 303 |
| Size | 24 | 20 | 124 |
