ssr_primer
Primer Details for TRINITY_DN10477_c0_g1_i5 |
|---|
| (AT)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTAGCCACGACAATGCCAA | GGCTTGTTTCGACGTTGCTT | Start: 1127 |
| Tm | 59.964 | 59.971 | End: 1232 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CCACCACTAGCCACGACAAT | GGCTTGTTTCGACGTTGCTT | Start: 1122 |
| Tm | 60.036 | 59.971 | End: 1232 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | TCCTTCCTGTCCTCCAACCA | CGATTCGTGCAAAGTAGCCG | Start: 621 |
| Tm | 60.105 | 59.975 | End: 851 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | CGGCTACTTTGCACGAATCG | TGGAGACTATTCGGCATGGC | Start: 832 |
| Tm | 59.975 | 59.893 | End: 1103 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | CGGCTACTTTGCACGAATCG | GCAAGCCACCAAGTACATGC | Start: 832 |
| Tm | 59.975 | 60.109 | End: 939 |
| Size | 20 | 20 | 108 |
(CTCCT)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTAGCCACGACAATGCCAA | GGCTTGTTTCGACGTTGCTT | Start: 1127 |
| Tm | 59.964 | 59.971 | End: 1232 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CCACCACTAGCCACGACAAT | GGCTTGTTTCGACGTTGCTT | Start: 1122 |
| Tm | 60.036 | 59.971 | End: 1232 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | TCCTTCCTGTCCTCCAACCA | CGATTCGTGCAAAGTAGCCG | Start: 621 |
| Tm | 60.105 | 59.975 | End: 851 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | CGGCTACTTTGCACGAATCG | TGGAGACTATTCGGCATGGC | Start: 832 |
| Tm | 59.975 | 59.893 | End: 1103 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | CGGCTACTTTGCACGAATCG | GCAAGCCACCAAGTACATGC | Start: 832 |
| Tm | 59.975 | 60.109 | End: 939 |
| Size | 20 | 20 | 108 |
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