Primer Details for TRINITY_DN10321_c3_g3_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACCTAGACTTCAAGATGGATGA | CGTTGGACACGGAAACCTAGA | Start: 81 |
| Tm | 58.476 | 60 | End: 213 |
| Size | 24 | 21 | 133 |
| Primer 2 details | |||
| Sequence | GACCTAGACTTCAAGATGGATGA | CGTTGGACACGGAAACCTAGA | Start: 82 |
| Tm | 57.139 | 60 | End: 213 |
| Size | 23 | 21 | 132 |
| Primer 3 details | |||
| Sequence | AGACCTAGACTTCAAGATGGATG | CGTTGGACACGGAAACCTAGA | Start: 81 |
| Tm | 57.132 | 60 | End: 213 |
| Size | 23 | 21 | 133 |
| Primer 4 details | |||
| Sequence | ACCTAGACTTCAAGATGGATGATGA | CGTTGGACACGGAAACCTAGA | Start: 83 |
| Tm | 59.098 | 60 | End: 213 |
| Size | 25 | 21 | 131 |
| Primer 5 details | |||
| Sequence | AGACCTAGACTTCAAGATGGATGA | CGTTGGACACGGAAACCTAG | Start: 81 |
| Tm | 58.476 | 58.575 | End: 213 |
| Size | 24 | 20 | 133 |
