ssr_primer
Primer Details for TRINITY_DN10321_c3_g2_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCACGCACTGTTTCAGGA | GTTGGACACGGAAACTAGGGT | Start: 173 |
| Tm | 59.823 | 59.929 | End: 370 |
| Size | 20 | 21 | 198 |
| Primer 2 details |
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| Sequence | GTGTCACGCACTGTTTCAGG | GTTGGACACGGAAACTAGGGT | Start: 172 |
| Tm | 59.695 | 59.929 | End: 370 |
| Size | 20 | 21 | 199 |
| Primer 3 details |
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| Sequence | TGTCACGCACTGTTTCAGGA | CGTTGGACACGGAAACTAGG | Start: 173 |
| Tm | 59.823 | 58.575 | End: 371 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | TGTCACGCACTGTTTCAGGA | TGGACACGGAAACTAGGGTT | Start: 173 |
| Tm | 59.823 | 58.573 | End: 368 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
|---|
| Sequence | TGTCACGCACTGTTTCAGGA | TTGGACACGGAAACTAGGGT | Start: 173 |
| Tm | 59.823 | 58.573 | End: 369 |
| Size | 20 | 20 | 197 |
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