Primer Details for TRINITY_DN10321_c2_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGTCAAACGTTAGACACAGA | AGTAATGGCATACTTTAAAACTGGTAA | Start: 45 |
| Tm | 59.9 | 57.477 | End: 183 |
| Size | 22 | 27 | 139 |
| Primer 2 details |
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| Sequence | ACGGTCAAACGTTAGACACAGA | ATAGTAATGGCATACTTTAAAACTGGT | Start: 45 |
| Tm | 59.9 | 57.305 | End: 185 |
| Size | 22 | 27 | 141 |
| Primer 3 details |
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| Sequence | ACGGTCAAACGTTAGACACAG | AGTAATGGCATACTTTAAAACTGGTAA | Start: 45 |
| Tm | 58.533 | 57.477 | End: 183 |
| Size | 21 | 27 | 139 |
| Primer 4 details |
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| Sequence | ACGGTCAAACGTTAGACACA | AGTAATGGCATACTTTAAAACTGGTAA | Start: 45 |
| Tm | 57.417 | 57.477 | End: 183 |
| Size | 20 | 27 | 139 |
| Primer 5 details |
|---|
| Sequence | ACGGTCAAACGTTAGACACAG | ATAGTAATGGCATACTTTAAAACTGGT | Start: 45 |
| Tm | 58.533 | 57.305 | End: 185 |
| Size | 21 | 27 | 141 |
