ssr_primer
Primer Details for TRINITY_DN10315_c1_g1_i7 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGGGTTATAGCGACGTGC | ACTATGCATGCACACCCGAA | Start: 652 |
| Tm | 59.971 | 60.036 | End: 904 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCATGGACCTTCGAAACCGG | GCACGTCGCTATAACCCGTA | Start: 481 |
| Tm | 60.037 | 59.971 | End: 671 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
|---|
| Sequence | TACGGGTTATAGCGACGTGC | GCTGTTCAAGAGGCTCTCGT | Start: 652 |
| Tm | 59.971 | 60.038 | End: 773 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | ACATGGCAGATGCATGTCCA | AACCTGTCATCTTCCGGCAG | Start: 1390 |
| Tm | 60.034 | 60.036 | End: 1594 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | ACATGGCAGATGCATGTCCA | TCAACCTGTCATCTTCCGGC | Start: 1390 |
| Tm | 60.034 | 60.036 | End: 1596 |
| Size | 20 | 20 | 207 |
(AT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGGGTTATAGCGACGTGC | ACTATGCATGCACACCCGAA | Start: 652 |
| Tm | 59.971 | 60.036 | End: 904 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCATGGACCTTCGAAACCGG | GCACGTCGCTATAACCCGTA | Start: 481 |
| Tm | 60.037 | 59.971 | End: 671 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
|---|
| Sequence | TACGGGTTATAGCGACGTGC | GCTGTTCAAGAGGCTCTCGT | Start: 652 |
| Tm | 59.971 | 60.038 | End: 773 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | ACATGGCAGATGCATGTCCA | AACCTGTCATCTTCCGGCAG | Start: 1390 |
| Tm | 60.034 | 60.036 | End: 1594 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | ACATGGCAGATGCATGTCCA | TCAACCTGTCATCTTCCGGC | Start: 1390 |
| Tm | 60.034 | 60.036 | End: 1596 |
| Size | 20 | 20 | 207 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGGGTTATAGCGACGTGC | ACTATGCATGCACACCCGAA | Start: 652 |
| Tm | 59.971 | 60.036 | End: 904 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCATGGACCTTCGAAACCGG | GCACGTCGCTATAACCCGTA | Start: 481 |
| Tm | 60.037 | 59.971 | End: 671 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
|---|
| Sequence | TACGGGTTATAGCGACGTGC | GCTGTTCAAGAGGCTCTCGT | Start: 652 |
| Tm | 59.971 | 60.038 | End: 773 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | ACATGGCAGATGCATGTCCA | AACCTGTCATCTTCCGGCAG | Start: 1390 |
| Tm | 60.034 | 60.036 | End: 1594 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | ACATGGCAGATGCATGTCCA | TCAACCTGTCATCTTCCGGC | Start: 1390 |
| Tm | 60.034 | 60.036 | End: 1596 |
| Size | 20 | 20 | 207 |
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