ssr_primer
Primer Details for TRINITY_DN10186_c0_g1_i6 |
|---|
| (GAA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTCTGTTTACAGCCGCAT | GATTGACGCACCGAATGACG | Start: 1370 |
| Tm | 60.036 | 59.975 | End: 1594 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
|---|
| Sequence | TGTTTACAGCCGCATCCACT | GATTGACGCACCGAATGACG | Start: 1375 |
| Tm | 59.964 | 59.975 | End: 1594 |
| Size | 20 | 20 | 220 |
| Primer 3 details |
|---|
| Sequence | GCGCCTAGAAATACCGCTCT | CCCAAATGCCGCAAAACAGA | Start: 874 |
| Tm | 59.968 | 59.967 | End: 1045 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | TCCCCTGCTAGAGCTCTGTT | GATTGACGCACCGAATGACG | Start: 1359 |
| Tm | 59.958 | 59.975 | End: 1594 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | TTCCCCTGCTAGAGCTCTGT | GATTGACGCACCGAATGACG | Start: 1358 |
| Tm | 59.958 | 59.975 | End: 1594 |
| Size | 20 | 20 | 237 |
(A)12gaagaaaaggaaaatggaaaagagagagggaggaagaaggagaaggactgtgaccgtatatgtgtattatggagaaagaagaatggaataaaatacag(AGA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTCTGTTTACAGCCGCAT | GATTGACGCACCGAATGACG | Start: 1370 |
| Tm | 60.036 | 59.975 | End: 1594 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
|---|
| Sequence | TGTTTACAGCCGCATCCACT | GATTGACGCACCGAATGACG | Start: 1375 |
| Tm | 59.964 | 59.975 | End: 1594 |
| Size | 20 | 20 | 220 |
| Primer 3 details |
|---|
| Sequence | GCGCCTAGAAATACCGCTCT | CCCAAATGCCGCAAAACAGA | Start: 874 |
| Tm | 59.968 | 59.967 | End: 1045 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | TCCCCTGCTAGAGCTCTGTT | GATTGACGCACCGAATGACG | Start: 1359 |
| Tm | 59.958 | 59.975 | End: 1594 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | TTCCCCTGCTAGAGCTCTGT | GATTGACGCACCGAATGACG | Start: 1358 |
| Tm | 59.958 | 59.975 | End: 1594 |
| Size | 20 | 20 | 237 |
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