ssr_primer
Primer Details for TRINITY_DN10013_c0_g1_i9 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCACATCGTTCGCAACAA | GATTCCCCCACGTCACAACT | Start: 392 |
| Tm | 60.041 | 59.964 | End: 650 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | AACCAAGGCCCACACTTGAA | TGTTGATTGGGTTTGGGGCT | Start: 2047 |
| Tm | 60.033 | 60.105 | End: 2241 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | AAACCAAGGCCCACACTTGA | TGTTGATTGGGTTTGGGGCT | Start: 2046 |
| Tm | 60.033 | 60.105 | End: 2241 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | AGCCCCAAACCCAATCAACA | GTTGGGGTGCTTGATGAGGA | Start: 2222 |
| Tm | 60.105 | 59.962 | End: 2474 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | AACCAAGGCCCACACTTGAA | TCTTTTGGAGCTGCATGGGT | Start: 2047 |
| Tm | 60.033 | 59.887 | End: 2168 |
| Size | 20 | 20 | 122 |
(AC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCACATCGTTCGCAACAA | GATTCCCCCACGTCACAACT | Start: 392 |
| Tm | 60.041 | 59.964 | End: 650 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | AACCAAGGCCCACACTTGAA | TGTTGATTGGGTTTGGGGCT | Start: 2047 |
| Tm | 60.033 | 60.105 | End: 2241 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | AAACCAAGGCCCACACTTGA | TGTTGATTGGGTTTGGGGCT | Start: 2046 |
| Tm | 60.033 | 60.105 | End: 2241 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | AGCCCCAAACCCAATCAACA | GTTGGGGTGCTTGATGAGGA | Start: 2222 |
| Tm | 60.105 | 59.962 | End: 2474 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | AACCAAGGCCCACACTTGAA | TCTTTTGGAGCTGCATGGGT | Start: 2047 |
| Tm | 60.033 | 59.887 | End: 2168 |
| Size | 20 | 20 | 122 |
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