ssr_primer
Primer Details for NODE_98105_length_526_cov_3.187638 |
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| (AG)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCAAATTCCGCCGACAA | GGTGGGGGTTTCCATTCGAT | Start: 230 |
| Tm | 59.964 | 60.034 | End: 406 |
| Size | 20 | 20 | 177 |
| Primer 2 details |
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| Sequence | TTGTCCATTGAGTGACGGGG | GGTGGGGGTTTCCATTCGAT | Start: 170 |
| Tm | 59.964 | 60.034 | End: 406 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
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| Sequence | ACATGTCCGGCACTGTAAGG | GGTGGGGGTTTCCATTCGAT | Start: 251 |
| Tm | 60.036 | 60.034 | End: 406 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
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| Sequence | TTGTCCATTGAGTGACGGGG | CCTTACAGTGCCGGACATGT | Start: 170 |
| Tm | 59.964 | 60.036 | End: 270 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
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| Sequence | GTACGGCGGACCACAAAAAC | GGTGGGGGTTTCCATTCGAT | Start: 140 |
| Tm | 60.041 | 60.034 | End: 406 |
| Size | 20 | 20 | 267 |
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