Primer Details for NODE_842_length_375_cov_1.450331 | |||
|---|---|---|---|
| (A)10tgtacaatttttatacgtacatttgttccacttttaattgttta(T)11aattaa(T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGGGGATGCATGAAGATT | AGATGTCTCAAAGGCATTCATTG | Start: 0 |
| Tm | 59.447 | 57.422 | End: 276 |
| Size | 20 | 23 | 277 |
| Primer 2 details | |||
| Sequence | ACGGGGGATGCATGAAGATT | AAGATGTCTCAAAGGCATTCATTG | Start: 0 |
| Tm | 59.447 | 58.038 | End: 277 |
| Size | 20 | 24 | 278 |
| Primer 3 details | |||
| Sequence | ACGGGGGATGCATGAAGATT | GCCAATGCAATAATTCTATTGAACGG | Start: 0 |
| Tm | 59.447 | 59.856 | End: 254 |
| Size | 20 | 26 | 255 |
| Primer 4 details | |||
| Sequence | CGGGGGATGCATGAAGATTCT | AGATGTCTCAAAGGCATTCATTG | Start: 1 |
| Tm | 60.203 | 57.422 | End: 276 |
| Size | 21 | 23 | 276 |
| Primer 5 details | |||
| Sequence | CGGGGGATGCATGAAGATTC | AGATGTCTCAAAGGCATTCATTG | Start: 1 |
| Tm | 58.758 | 57.422 | End: 276 |
| Size | 20 | 23 | 276 |
