Primer Details for NODE_78759_length_227_cov_7.233766 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAGAGCAAACCAGAGTGT | TGGATAAAATTGGATAAGCGAGACA | Start: 38 |
| Tm | 59.819 | 58.647 | End: 193 |
| Size | 20 | 25 | 156 |
| Primer 2 details | |||
| Sequence | TGCAGAGCAAACCAGAGTGT | TTGGATAAGCGAGACAAAAGAAAA | Start: 38 |
| Tm | 59.819 | 57.373 | End: 184 |
| Size | 20 | 24 | 147 |
| Primer 3 details | |||
| Sequence | GCAAGATGCAGAGCAAACCA | TGGATAAAATTGGATAAGCGAGACA | Start: 32 |
| Tm | 59.4 | 58.647 | End: 193 |
| Size | 20 | 25 | 162 |
| Primer 4 details | |||
| Sequence | TGCAGAGCAAACCAGAGTGT | TGGATAAGCGAGACAAAAGAAAAT | Start: 38 |
| Tm | 59.819 | 57.182 | End: 183 |
| Size | 20 | 24 | 146 |
| Primer 5 details | |||
| Sequence | TGCAGAGCAAACCAGAGTGT | ATTGGATAAGCGAGACAAAAGAAA | Start: 38 |
| Tm | 59.819 | 57.182 | End: 185 |
| Size | 20 | 24 | 148 |
