ssr_primer
Primer Details for NODE_75856_length_610_cov_3.150838 |
|---|
| (TGC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTCCCCAGTTTAGCAGCA | AAAGACTAGCAGCAGCAGCA | Start: 81 |
| Tm | 59.963 | 59.964 | End: 276 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | AGCAGCAACACCTTAGGACC | AAAGACTAGCAGCAGCAGCA | Start: 94 |
| Tm | 59.963 | 59.964 | End: 276 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | TGCTGCTGCTGCTAGTCTTT | GCCAGCTTGCTTACAAACCC | Start: 257 |
| Tm | 59.964 | 60.038 | End: 406 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | TGCTGCTGCTGCTAGTCTTT | ACCACTTATGCCAGCTTGCT | Start: 257 |
| Tm | 59.964 | 59.961 | End: 415 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GGGTTTGTAAGCAAGCTGGC | TCCGGCTGAGATTTGTCACC | Start: 387 |
| Tm | 60.038 | 60.036 | End: 579 |
| Size | 20 | 20 | 193 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTCCCCAGTTTAGCAGCA | AAAGACTAGCAGCAGCAGCA | Start: 81 |
| Tm | 59.963 | 59.964 | End: 276 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | AGCAGCAACACCTTAGGACC | AAAGACTAGCAGCAGCAGCA | Start: 94 |
| Tm | 59.963 | 59.964 | End: 276 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | TGCTGCTGCTGCTAGTCTTT | GCCAGCTTGCTTACAAACCC | Start: 257 |
| Tm | 59.964 | 60.038 | End: 406 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | TGCTGCTGCTGCTAGTCTTT | ACCACTTATGCCAGCTTGCT | Start: 257 |
| Tm | 59.964 | 59.961 | End: 415 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GGGTTTGTAAGCAAGCTGGC | TCCGGCTGAGATTTGTCACC | Start: 387 |
| Tm | 60.038 | 60.036 | End: 579 |
| Size | 20 | 20 | 193 |
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