ssr_primer
Primer Details for NODE_72225_length_1683_cov_4.319255 |
|---|
| (A)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTATTCGTGGTGCACCTGCT | GTCTCTTCAGGCCCATCTCG | Start: 205 |
| Tm | 59.964 | 59.896 | End: 439 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | ACTCAACAAAGCGCTCCAGA | GTGAGTCCGTTGGTAGCCAA | Start: 870 |
| Tm | 59.893 | 59.966 | End: 1128 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | GTCCCTGGCCAGAATCAACA | GTCTCTTCAGGCCCATCTCG | Start: 171 |
| Tm | 59.962 | 59.896 | End: 439 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
|---|
| Sequence | CTCCATTTCGAACTCCGGCT | GTGAGTCCGTTGGTAGCCAA | Start: 929 |
| Tm | 60.108 | 59.966 | End: 1128 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
|---|
| Sequence | ACTCAACAAAGCGCTCCAGA | TCAGCAAGCAGTGGAGAAGG | Start: 870 |
| Tm | 59.893 | 59.964 | End: 1093 |
| Size | 20 | 20 | 224 |
(A)11tcgaatatgaatt(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTATTCGTGGTGCACCTGCT | GTCTCTTCAGGCCCATCTCG | Start: 205 |
| Tm | 59.964 | 59.896 | End: 439 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | ACTCAACAAAGCGCTCCAGA | GTGAGTCCGTTGGTAGCCAA | Start: 870 |
| Tm | 59.893 | 59.966 | End: 1128 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | GTCCCTGGCCAGAATCAACA | GTCTCTTCAGGCCCATCTCG | Start: 171 |
| Tm | 59.962 | 59.896 | End: 439 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
|---|
| Sequence | CTCCATTTCGAACTCCGGCT | GTGAGTCCGTTGGTAGCCAA | Start: 929 |
| Tm | 60.108 | 59.966 | End: 1128 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
|---|
| Sequence | ACTCAACAAAGCGCTCCAGA | TCAGCAAGCAGTGGAGAAGG | Start: 870 |
| Tm | 59.893 | 59.964 | End: 1093 |
| Size | 20 | 20 | 224 |
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