Primer Details for NODE_72105_length_270_cov_4.121827 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTAATGAAGGAAGGCTTTGC | TGACCATGATCTGTAAGGATCTGT | Start: 8 |
| Tm | 57.209 | 59.035 | End: 266 |
| Size | 22 | 24 | 259 |
| Primer 2 details | |||
| Sequence | AAAGTAATGAAGGAAGGCTTTGC | TGACCATGATCTGTAAGGATCTGT | Start: 7 |
| Tm | 57.861 | 59.035 | End: 266 |
| Size | 23 | 24 | 260 |
| Primer 3 details | |||
| Sequence | AAGTAATGAAGGAAGGCTTTGC | TTGACCATGATCTGTAAGGATCTGT | Start: 8 |
| Tm | 57.209 | 59.573 | End: 267 |
| Size | 22 | 25 | 260 |
| Primer 4 details | |||
| Sequence | AAGTAATGAAGGAAGGCTTTGC | TGACCATGATCTGTAAGGATCTG | Start: 8 |
| Tm | 57.209 | 57.458 | End: 266 |
| Size | 22 | 23 | 259 |
| Primer 5 details | |||
| Sequence | AAGTAATGAAGGAAGGCTTTGC | GACCATGATCTGTAAGGATCTGT | Start: 8 |
| Tm | 57.209 | 57.456 | End: 265 |
| Size | 22 | 23 | 258 |
