Primer Details for NODE_7031_length_270_cov_68.685279 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCTCAAACAATGATTTAGTGACT | TGAGTTTGACGGATCCGACA | Start: 16 |
| Tm | 59.23 | 59.037 | End: 164 |
| Size | 25 | 20 | 149 |
| Primer 2 details |
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| Sequence | TGTCGGATCCGTCAAACTCA | AGGTGTTTTCATTGATTCACACA | Start: 145 |
| Tm | 59.037 | 57.149 | End: 247 |
| Size | 20 | 23 | 103 |
| Primer 3 details |
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| Sequence | TGTCGGATCCGTCAAACTCA | CCTGAAGGTGTTTTCATTGATTCAC | Start: 145 |
| Tm | 59.037 | 59.077 | End: 252 |
| Size | 20 | 25 | 108 |
| Primer 4 details |
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| Sequence | TGGCTCAAACAATGATTTAGTGAC | TGAGTTTGACGGATCCGACA | Start: 16 |
| Tm | 57.987 | 59.037 | End: 164 |
| Size | 24 | 20 | 149 |
| Primer 5 details |
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| Sequence | GGCTCAAACAATGATTTAGTGACTCA | TGAGTTTGACGGATCCGACA | Start: 17 |
| Tm | 60.073 | 59.037 | End: 164 |
| Size | 26 | 20 | 148 |
