Primer Details for NODE_69258_length_247_cov_2.517241 |
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| (T)11gatttggtctctcaaaaaatttaactctgttattcctaagttaac(T)12cttttttaaggatctttttttctttttttttcttttttcgtttttattcgtttttaaggatcttttcatcgagttatca(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAACATCGAGGTAGCAATTTTT | ACTCGATGAAAAGATCCTTAAAAACGA | Start: 11 |
| Tm | 57.042 | 59.613 | End: 170 |
| Size | 23 | 27 | 160 |
| Primer 2 details |
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| Sequence | TTCAACATCGAGGTAGCAATTTT | ACTCGATGAAAAGATCCTTAAAAACGA | Start: 10 |
| Tm | 57.042 | 59.613 | End: 170 |
| Size | 23 | 27 | 161 |
| Primer 3 details |
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| Sequence | TCTTAATTCAACATCGAGGTAGCA | ACTCGATGAAAAGATCCTTAAAAACGA | Start: 4 |
| Tm | 58.027 | 59.613 | End: 170 |
| Size | 24 | 27 | 167 |
| Primer 4 details |
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| Sequence | TCAACATCGAGGTAGCAATTTTT | ACTCGATGAAAAGATCCTTAAAAACG | Start: 11 |
| Tm | 57.042 | 58.481 | End: 170 |
| Size | 23 | 26 | 160 |
| Primer 5 details |
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| Sequence | TTCAACATCGAGGTAGCAATTTT | ACTCGATGAAAAGATCCTTAAAAACG | Start: 10 |
| Tm | 57.042 | 58.481 | End: 170 |
| Size | 23 | 26 | 161 |
