Primer Details for NODE_66479_length_252_cov_1140.111732 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTACAAAGAACAAAAGATCCTT | TCGAGGTACATCGAGGTAGCA | Start: 66 |
| Tm | 57.04 | 60.134 | End: 246 |
| Size | 24 | 21 | 181 |
| Primer 2 details | |||
| Sequence | TTGCTACAAAGAACAAAAGATCCT | TCGAGGTACATCGAGGTAGCA | Start: 65 |
| Tm | 57.04 | 60.134 | End: 246 |
| Size | 24 | 21 | 182 |
| Primer 3 details | |||
| Sequence | TGCTACAAAGAACAAAAGATCCTT | CGAGGTACATCGAGGTAGCA | Start: 66 |
| Tm | 57.04 | 58.694 | End: 245 |
| Size | 24 | 20 | 180 |
| Primer 4 details | |||
| Sequence | TTGCTACAAAGAACAAAAGATCCT | CGAGGTACATCGAGGTAGCA | Start: 65 |
| Tm | 57.04 | 58.694 | End: 245 |
| Size | 24 | 20 | 181 |
| Primer 5 details | |||
| Sequence | TTGCTACAAAGAACAAAAGATCCTT | TCGAGGTACATCGAGGTAGCA | Start: 65 |
| Tm | 57.653 | 60.134 | End: 246 |
| Size | 25 | 21 | 182 |
