Primer Details for NODE_63793_length_204_cov_26.465649 | |||
|---|---|---|---|
| (T)10g(T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTTCTGCTTTTGATCCTGGT | TGAATTCAAGTAGGTGTTGTCACA | Start: 3 |
| Tm | 58.163 | 58.567 | End: 189 |
| Size | 22 | 24 | 187 |
| Primer 2 details | |||
| Sequence | TCTGCTTTTGATCCTGGTTTTGA | TGAATTCAAGTAGGTGTTGTCACA | Start: 7 |
| Tm | 58.786 | 58.567 | End: 189 |
| Size | 23 | 24 | 183 |
| Primer 3 details | |||
| Sequence | AGTTTCTGCTTTTGATCCTGGTT | TGAATTCAAGTAGGTGTTGTCACA | Start: 3 |
| Tm | 58.782 | 58.567 | End: 189 |
| Size | 23 | 24 | 187 |
| Primer 4 details | |||
| Sequence | AGTTTCTGCTTTTGATCCTGGT | TTGAATTCAAGTAGGTGTTGTCACA | Start: 3 |
| Tm | 58.163 | 59.117 | End: 190 |
| Size | 22 | 25 | 188 |
| Primer 5 details | |||
| Sequence | AGTTTCTGCTTTTGATCCTGGT | CTTGAATTCAAGTAGGTGTTGTCACA | Start: 3 |
| Tm | 58.163 | 59.962 | End: 191 |
| Size | 22 | 26 | 189 |
