ssr_primer
Primer Details for NODE_56469_length_540_cov_2.173448 |
|---|
| (AGC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCGAGAAGATCACCTCTC | TGTCGAACAGGGTCAACGAG | Start: 220 |
| Tm | 59.97 | 59.968 | End: 347 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | CGACTCTGTCACCAACGACA | TGTCGAACAGGGTCAACGAG | Start: 142 |
| Tm | 59.97 | 59.968 | End: 347 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
|---|
| Sequence | TCACCAACGACACCGAGAAG | TGTCGAACAGGGTCAACGAG | Start: 150 |
| Tm | 59.968 | 59.968 | End: 347 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
|---|
| Sequence | CCGCGAGAAGATCACCTCTC | AAGATTGGGACTCGGCCTTG | Start: 220 |
| Tm | 59.97 | 60.035 | End: 380 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
|---|
| Sequence | ACCTCTCTCCGTGAGCTGAT | TGTCGAACAGGGTCAACGAG | Start: 233 |
| Tm | 60.034 | 59.968 | End: 347 |
| Size | 20 | 20 | 115 |
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