Primer Details for NODE_52398_length_220_cov_7.360544 |
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| (T)10cgatttggtctctcaaaaaatttaactttgttatccctaaggtaactttttc(T)14aggatcttttgtttttgtagcaa(T)12gtttttttttgatttggtctcatttggtctcgtagcaa(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGATCTTTTGTTTTTGTAGCAATTT | AAGTTACCTTAGGGATAACAGAGTT | Start: 81 |
| Tm | 57.037 | 57.19 | End: 212 |
| Size | 26 | 25 | 132 |
| Primer 2 details |
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| Sequence | AGGATCTTTTGTTTTTGTAGCAATTT | AAAGTTACCTTAGGGATAACAGAGT | Start: 81 |
| Tm | 57.037 | 57.19 | End: 213 |
| Size | 26 | 25 | 133 |
| Primer 3 details |
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| Sequence | AGGATCTTTTGTTTTTGTAGCAATTT | AAAGTTACCTTAGGGATAACAGAGTT | Start: 81 |
| Tm | 57.037 | 57.784 | End: 213 |
| Size | 26 | 26 | 133 |
| Primer 4 details |
|---|
| Sequence | AGGATCTTTTGTTTTTGTAGCAATTT | AAAAGTTACCTTAGGGATAACAGAGT | Start: 81 |
| Tm | 57.037 | 57.784 | End: 214 |
| Size | 26 | 26 | 134 |
| Primer 5 details |
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| Sequence | AGGATCTTTTGTTTTTGTAGCAATTTT | AAGTTACCTTAGGGATAACAGAGTT | Start: 81 |
| Tm | 57.605 | 57.19 | End: 212 |
| Size | 27 | 25 | 132 |
