ssr_primer
Primer Details for NODE_51582_length_1356_cov_9.063133 |
|---|
| (TGC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAGCCTGATTTGCTCCGT | TAATGGAAACCCATGCGGCT | Start: 624 |
| Tm | 59.963 | 60.034 | End: 883 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
|---|
| Sequence | GCGCGCAGAATAATTCAGGG | GGGTCCAGGACACGGTAAAG | Start: 196 |
| Tm | 60.041 | 60.037 | End: 322 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | CTTTACCGTGTCCTGGACCC | CCCAGGAGTGTTTGGTTGGT | Start: 303 |
| Tm | 60.037 | 60.106 | End: 528 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | ACCAACCAAACACTCCTGGG | ACGGAGCAAATCAGGCTTCA | Start: 509 |
| Tm | 60.106 | 59.963 | End: 643 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CTTTACCGTGTCCTGGACCC | CAGCTCTTGTCCATCCCAGG | Start: 303 |
| Tm | 60.037 | 60.107 | End: 542 |
| Size | 20 | 20 | 240 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAGCCTGATTTGCTCCGT | TAATGGAAACCCATGCGGCT | Start: 624 |
| Tm | 59.963 | 60.034 | End: 883 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
|---|
| Sequence | GCGCGCAGAATAATTCAGGG | GGGTCCAGGACACGGTAAAG | Start: 196 |
| Tm | 60.041 | 60.037 | End: 322 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | CTTTACCGTGTCCTGGACCC | CCCAGGAGTGTTTGGTTGGT | Start: 303 |
| Tm | 60.037 | 60.106 | End: 528 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | ACCAACCAAACACTCCTGGG | ACGGAGCAAATCAGGCTTCA | Start: 509 |
| Tm | 60.106 | 59.963 | End: 643 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CTTTACCGTGTCCTGGACCC | CAGCTCTTGTCCATCCCAGG | Start: 303 |
| Tm | 60.037 | 60.107 | End: 542 |
| Size | 20 | 20 | 240 |
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