Primer Details for NODE_49212_length_214_cov_397.567376 |
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| (A)10t(A)11tatataattagtaaaaaaaag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCGGCAAATAAAATTATCCGAA | ATTTTATTTGCCGAGTTCCTAATTTTT | Start: 6 |
| Tm | 57.313 | 57.281 | End: 174 |
| Size | 24 | 27 | 169 |
| Primer 2 details |
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| Sequence | TCGGCAAATAAAATTATCCGAATGT | ATTTTATTTGCCGAGTTCCTAATTTTT | Start: 8 |
| Tm | 57.966 | 57.281 | End: 174 |
| Size | 25 | 27 | 167 |
| Primer 3 details |
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| Sequence | TCGGCAAATAAAATTATCCGAATGT | AATTTTATTTGCCGAGTTCCTAATTTT | Start: 8 |
| Tm | 57.966 | 57.281 | End: 175 |
| Size | 25 | 27 | 168 |
| Primer 4 details |
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| Sequence | CTCGGCAAATAAAATTATCCGAATGT | ATTTTATTTGCCGAGTTCCTAATTTTT | Start: 7 |
| Tm | 58.861 | 57.281 | End: 174 |
| Size | 26 | 27 | 168 |
| Primer 5 details |
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| Sequence | CTCGGCAAATAAAATTATCCGAATGT | AATTTTATTTGCCGAGTTCCTAATTTT | Start: 7 |
| Tm | 58.861 | 57.281 | End: 175 |
| Size | 26 | 27 | 169 |
