Primer Details for NODE_474958_length_204_cov_555.885496 | |||
|---|---|---|---|
| (A)19gttaccttagggataccagagttaaattttttgagagaccaaatcg(A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAACAAAAGATCCCAAAAGATCCT | TCAACATCGAGGTAGCAATTTTT | Start: 79 |
| Tm | 58.682 | 57.042 | End: 197 |
| Size | 25 | 23 | 119 |
| Primer 2 details | |||
| Sequence | AGAACAAAAGATCCCAAAAGATCCT | TTCAACATCGAGGTAGCAATTTT | Start: 79 |
| Tm | 58.682 | 57.042 | End: 198 |
| Size | 25 | 23 | 120 |
| Primer 3 details | |||
| Sequence | GAACAAAAGATCCCAAAAGATCCT | TCAACATCGAGGTAGCAATTTTT | Start: 80 |
| Tm | 57.404 | 57.042 | End: 197 |
| Size | 24 | 23 | 118 |
| Primer 4 details | |||
| Sequence | GAACAAAAGATCCCAAAAGATCCT | TTCAACATCGAGGTAGCAATTTT | Start: 80 |
| Tm | 57.404 | 57.042 | End: 198 |
| Size | 24 | 23 | 119 |
| Primer 5 details | |||
| Sequence | AGAACAAAAGATCCCAAAAGATCC | TCAACATCGAGGTAGCAATTTTT | Start: 79 |
| Tm | 57.404 | 57.042 | End: 197 |
| Size | 24 | 23 | 119 |
