Primer Details for NODE_474598_length_200_cov_79.314961 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGGATCTTTTGTTATCTTTTGTTCT | AAGTTACCTTAGGGATAACAGAGTT | Start: 72 |
| Tm | 57.645 | 57.19 | End: 174 |
| Size | 27 | 25 | 103 |
| Primer 2 details |
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| Sequence | TGAGGATCTTTTGTTATCTTTTGTTCT | AAAGTTACCTTAGGGATAACAGAGT | Start: 72 |
| Tm | 57.645 | 57.19 | End: 175 |
| Size | 27 | 25 | 104 |
| Primer 3 details |
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| Sequence | TGAGGATCTTTTGTTATCTTTTGTTCT | AAAGTTACCTTAGGGATAACAGAGTT | Start: 72 |
| Tm | 57.645 | 57.784 | End: 175 |
| Size | 27 | 26 | 104 |
| Primer 4 details |
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| Sequence | TGAGGATCTTTTGTTATCTTTTGTTCT | AAAAGTTACCTTAGGGATAACAGAGT | Start: 72 |
| Tm | 57.645 | 57.784 | End: 176 |
| Size | 27 | 26 | 105 |
| Primer 5 details |
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| Sequence | TTGAGGATCTTTTGTTATCTTTTGTTC | AAGTTACCTTAGGGATAACAGAGTT | Start: 71 |
| Tm | 57.034 | 57.19 | End: 174 |
| Size | 27 | 25 | 104 |
