Primer Details for NODE_474295_length_200_cov_12.614173 |
|---|
| (A)10ttgctacctcgatgaaaccgaaaaaaaccg(A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTGAGAGACCAAACCGAAAAA | AGGATTTTTAATCGAGGTAGCAATT | Start: 31 |
| Tm | 57.353 | 57.118 | End: 131 |
| Size | 23 | 25 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTTGAGAGACCAAACCGAAAAA | AAGGATTTTTAATCGAGGTAGCAAT | Start: 31 |
| Tm | 57.353 | 57.118 | End: 132 |
| Size | 23 | 25 | 102 |
| Primer 3 details |
|---|
| Sequence | TTTTTGAGAGACCAAACCGAAAA | AGGATTTTTAATCGAGGTAGCAATT | Start: 30 |
| Tm | 57.353 | 57.118 | End: 131 |
| Size | 23 | 25 | 102 |
| Primer 4 details |
|---|
| Sequence | TTTTTGAGAGACCAAACCGAAAA | AAGGATTTTTAATCGAGGTAGCAAT | Start: 30 |
| Tm | 57.353 | 57.118 | End: 132 |
| Size | 23 | 25 | 103 |
| Primer 5 details |
|---|
| Sequence | TTTTTGAGAGACCAAACCGAAAAA | AGGATTTTTAATCGAGGTAGCAATT | Start: 30 |
| Tm | 57.973 | 57.118 | End: 131 |
| Size | 24 | 25 | 102 |
