Primer Details for NODE_468475_length_377_cov_5.457237 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACATTTCACAACATTTCACTTTCTT | TTGCAACTTGAACACAGCTTGA | Start: 20 |
| Tm | 57.224 | 59.509 | End: 119 |
| Size | 26 | 22 | 100 |
| Primer 2 details |
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| Sequence | AACATTTCACAACATTTCACTTTCTT | TTGCAACTTGAACACAGCTTG | Start: 20 |
| Tm | 57.224 | 58.121 | End: 119 |
| Size | 26 | 21 | 100 |
| Primer 3 details |
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| Sequence | AACATTTCACAACATTTCACTTTCTTT | TTGCAACTTGAACACAGCTTGA | Start: 20 |
| Tm | 57.782 | 59.509 | End: 119 |
| Size | 27 | 22 | 100 |
| Primer 4 details |
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| Sequence | ACATTTCACAACATTTCACTTTCTTT | ATTGCAACTTGAACACAGCTTGA | Start: 21 |
| Tm | 57.224 | 59.869 | End: 120 |
| Size | 26 | 23 | 100 |
| Primer 5 details |
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| Sequence | ACATTTCACAACATTTCACTTTCTTT | TGAATTGCAACTTGAACACAGCT | Start: 21 |
| Tm | 57.224 | 59.869 | End: 123 |
| Size | 26 | 23 | 103 |
