Primer Details for NODE_452924_length_209_cov_1.779412 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTAACTGTTTGTTTAAAGTAGTGAAG | TCGAGAAGGGCAGAAAAACCT | Start: 62 |
| Tm | 57.288 | 59.58 | End: 161 |
| Size | 27 | 21 | 100 |
| Primer 2 details |
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| Sequence | ACTGTTTGTTTAAAGTAGTGAAGGAA | CTCATAACTCGAGAAGGGCAGA | Start: 66 |
| Tm | 57.43 | 59.31 | End: 169 |
| Size | 26 | 22 | 104 |
| Primer 3 details |
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| Sequence | ACTGTTTGTTTAAAGTAGTGAAGGAA | TCTCATAACTCGAGAAGGGCAG | Start: 66 |
| Tm | 57.43 | 59.31 | End: 170 |
| Size | 26 | 22 | 105 |
| Primer 4 details |
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| Sequence | AACTGTTTGTTTAAAGTAGTGAAGGA | CTCATAACTCGAGAAGGGCAGA | Start: 65 |
| Tm | 57.43 | 59.31 | End: 169 |
| Size | 26 | 22 | 105 |
| Primer 5 details |
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| Sequence | AACTGTTTGTTTAAAGTAGTGAAGGA | TCTCATAACTCGAGAAGGGCAG | Start: 65 |
| Tm | 57.43 | 59.31 | End: 170 |
| Size | 26 | 22 | 106 |
