Primer Details for NODE_449033_length_203_cov_1.892308 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTACCTTAGCTTAAAAAGGCTGTT | GGAACTCGGCAAATAAAATTATCCG | Start: 16 |
| Tm | 57.181 | 59.087 | End: 128 |
| Size | 24 | 25 | 113 |
| Primer 2 details |
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| Sequence | CTACCTTAGCTTAAAAAGGCTGTT | GGAACTCGGCAAATAAAATTATCCGA | Start: 16 |
| Tm | 57.181 | 60.239 | End: 128 |
| Size | 24 | 26 | 113 |
| Primer 3 details |
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| Sequence | CTACCTTAGCTTAAAAAGGCTGTT | AGGAACTCGGCAAATAAAATTATCCG | Start: 16 |
| Tm | 57.181 | 60.239 | End: 129 |
| Size | 24 | 26 | 114 |
| Primer 4 details |
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| Sequence | AAAGGCTGTTAAATATTTCACTGAGCA | AGGAACTCGGCAAATAAAATTATCCG | Start: 30 |
| Tm | 60.04 | 60.239 | End: 129 |
| Size | 27 | 26 | 100 |
| Primer 5 details |
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| Sequence | ACCTTAGCTTAAAAAGGCTGTTAAA | GGAACTCGGCAAATAAAATTATCCG | Start: 18 |
| Tm | 57.409 | 59.087 | End: 128 |
| Size | 25 | 25 | 111 |
