Primer Details for NODE_448801_length_239_cov_2.361446 | |||
|---|---|---|---|
| (T)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGATAAAGCCATGTAAAGCCA | TCAACAATAGCTTTCAGGGGAA | Start: 97 |
| Tm | 58.401 | 57.619 | End: 238 |
| Size | 23 | 22 | 142 |
| Primer 2 details | |||
| Sequence | ACTGATAAAGCCATGTAAAGCCA | TCAACAATAGCTTTCAGGGGAAA | Start: 97 |
| Tm | 58.401 | 58.265 | End: 238 |
| Size | 23 | 23 | 142 |
| Primer 3 details | |||
| Sequence | TGATAAAGCCATGTAAAGCCATGT | TCAACAATAGCTTTCAGGGGAA | Start: 99 |
| Tm | 59.045 | 57.619 | End: 238 |
| Size | 24 | 22 | 140 |
| Primer 4 details | |||
| Sequence | AACTGATAAAGCCATGTAAAGCCA | TCAACAATAGCTTTCAGGGGAA | Start: 96 |
| Tm | 58.984 | 57.619 | End: 238 |
| Size | 24 | 22 | 143 |
| Primer 5 details | |||
| Sequence | CTGATAAAGCCATGTAAAGCCATGT | TCAACAATAGCTTTCAGGGGAA | Start: 98 |
| Tm | 59.932 | 57.619 | End: 238 |
| Size | 25 | 22 | 141 | (T)14: Primer 1 details |
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGATAAAGCCATGTAAAGCCA | TCAACAATAGCTTTCAGGGGAA | Start: 97 |
| Tm | 58.401 | 57.619 | End: 238 |
| Size | 23 | 22 | 142 |
| Primer 2 details | |||
| Sequence | ACTGATAAAGCCATGTAAAGCCA | TCAACAATAGCTTTCAGGGGAAA | Start: 97 |
| Tm | 58.401 | 58.265 | End: 238 |
| Size | 23 | 23 | 142 |
| Primer 3 details | |||
| Sequence | TGATAAAGCCATGTAAAGCCATGT | TCAACAATAGCTTTCAGGGGAA | Start: 99 |
| Tm | 59.045 | 57.619 | End: 238 |
| Size | 24 | 22 | 140 |
| Primer 4 details | |||
| Sequence | AACTGATAAAGCCATGTAAAGCCA | TCAACAATAGCTTTCAGGGGAA | Start: 96 |
| Tm | 58.984 | 57.619 | End: 238 |
| Size | 24 | 22 | 143 |
| Primer 5 details | |||
| Sequence | CTGATAAAGCCATGTAAAGCCATGT | TCAACAATAGCTTTCAGGGGAA | Start: 98 |
| Tm | 59.932 | 57.619 | End: 238 |
| Size | 25 | 22 | 141 |
