Primer Details for NODE_427548_length_257_cov_1.853261 |
|---|
| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCTTAAGAAGCCCTGCAAA | TGAATGACTGTTAAGAGTTATACGGA | Start: 6 |
| Tm | 57.491 | 57.941 | End: 132 |
| Size | 21 | 26 | 127 |
| Primer 2 details |
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| Sequence | TGATACTCTTAAGAAGCCCTGCA | TGAATGACTGTTAAGAGTTATACGGA | Start: 2 |
| Tm | 59.225 | 57.941 | End: 132 |
| Size | 23 | 26 | 131 |
| Primer 3 details |
|---|
| Sequence | ACTCTTAAGAAGCCCTGCAAAA | TGAATGACTGTTAAGAGTTATACGGA | Start: 6 |
| Tm | 58.167 | 57.941 | End: 132 |
| Size | 22 | 26 | 127 |
| Primer 4 details |
|---|
| Sequence | ACTCTTAAGAAGCCCTGCAAA | TTGAATGACTGTTAAGAGTTATACGGA | Start: 6 |
| Tm | 57.491 | 58.475 | End: 133 |
| Size | 21 | 27 | 128 |
| Primer 5 details |
|---|
| Sequence | ACTCTTAAGAAGCCCTGCAAA | AGTGATTTGAATGACTGTTAAGAGTT | Start: 6 |
| Tm | 57.491 | 57.317 | End: 139 |
| Size | 21 | 26 | 134 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCTTAAGAAGCCCTGCAAA | TGAATGACTGTTAAGAGTTATACGGA | Start: 6 |
| Tm | 57.491 | 57.941 | End: 132 |
| Size | 21 | 26 | 127 |
| Primer 2 details |
|---|
| Sequence | TGATACTCTTAAGAAGCCCTGCA | TGAATGACTGTTAAGAGTTATACGGA | Start: 2 |
| Tm | 59.225 | 57.941 | End: 132 |
| Size | 23 | 26 | 131 |
| Primer 3 details |
|---|
| Sequence | ACTCTTAAGAAGCCCTGCAAAA | TGAATGACTGTTAAGAGTTATACGGA | Start: 6 |
| Tm | 58.167 | 57.941 | End: 132 |
| Size | 22 | 26 | 127 |
| Primer 4 details |
|---|
| Sequence | ACTCTTAAGAAGCCCTGCAAA | TTGAATGACTGTTAAGAGTTATACGGA | Start: 6 |
| Tm | 57.491 | 58.475 | End: 133 |
| Size | 21 | 27 | 128 |
| Primer 5 details |
|---|
| Sequence | ACTCTTAAGAAGCCCTGCAAA | AGTGATTTGAATGACTGTTAAGAGTT | Start: 6 |
| Tm | 57.491 | 57.317 | End: 139 |
| Size | 21 | 26 | 134 |
