ssr_primer
Primer Details for NODE_423299_length_470_cov_5.030227 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAAGCAAAATGGGGGCAGG | TTCAGTTATGCCCAACCCCC | Start: 134 |
| Tm | 59.599 | 59.96 | End: 383 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
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| Sequence | AAAAGCAAAATGGGGGCAGG | CAGTTATGCCCAACCCCCTT | Start: 134 |
| Tm | 59.599 | 59.959 | End: 381 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
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| Sequence | GAAGGGGGTTGGGCATAACT | AATTGGTCTGCCTTCCAGCA | Start: 361 |
| Tm | 59.665 | 59.887 | End: 460 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | ACAGGGGCATCCACAATTGT | CCTGCCCCCATTTTGCTTTT | Start: 47 |
| Tm | 59.885 | 59.599 | End: 153 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
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| Sequence | AACAAAAGAGGGGGAAGGGG | TTGGTCTGCCTTCCAGCAAA | Start: 348 |
| Tm | 59.513 | 60.106 | End: 458 |
| Size | 20 | 20 | 111 |
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