Primer Details for NODE_418259_length_210_cov_1.467153 | |||
|---|---|---|---|
| (T)22: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATCCTGGTTTTGAAACAATGT | ACCAGGATCAAAAGCAGAAACT | Start: 27 |
| Tm | 57.132 | 58.163 | End: 186 |
| Size | 23 | 22 | 160 |
| Primer 2 details | |||
| Sequence | AGTTTCTGCTTTTGATCCTGGT | ACATTGTTTCAAAACCAGGATCA | Start: 15 |
| Tm | 58.163 | 57.132 | End: 199 |
| Size | 22 | 23 | 185 |
| Primer 3 details | |||
| Sequence | TCCTGGTTTTGAAACAATGTTCT | ACCAGGATCAAAAGCAGAAACT | Start: 30 |
| Tm | 57.069 | 58.163 | End: 186 |
| Size | 23 | 22 | 157 |
| Primer 4 details | |||
| Sequence | AGTTTCTGCTTTTGATCCTGGT | AGAACATTGTTTCAAAACCAGGA | Start: 15 |
| Tm | 58.163 | 57.069 | End: 202 |
| Size | 22 | 23 | 188 |
| Primer 5 details | |||
| Sequence | TGATCCTGGTTTTGAAACAATGTTCT | ACCAGGATCAAAAGCAGAAACT | Start: 27 |
| Tm | 59.902 | 58.163 | End: 186 |
| Size | 26 | 22 | 160 |
