ssr_primer
Primer Details for NODE_383187_length_858_cov_1.463694 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTGATGCACAGGGTAGAT | CGCAATGGGACTCTGGAAGT | Start: 623 |
| Tm | 59.534 | 60.036 | End: 826 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
|---|
| Sequence | ACTATCGATGCGGTGGGTTT | CTACCCTGTGCATCACGGTT | Start: 447 |
| Tm | 59.46 | 60.036 | End: 640 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
|---|
| Sequence | ACTATCGATGCGGTGGGTTT | TACCCTGTGCATCACGGTTC | Start: 447 |
| Tm | 59.46 | 60.037 | End: 639 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
|---|
| Sequence | CCGTGATGCACAGGGTAGAT | TCGCAATGGGACTCTGGAAG | Start: 623 |
| Tm | 59.534 | 59.75 | End: 827 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | ACTATCGATGCGGTGGGTTT | ATCTACCCTGTGCATCACGG | Start: 447 |
| Tm | 59.46 | 59.534 | End: 642 |
| Size | 20 | 20 | 196 |
(T)11atttttttacttgtcaaacgctttactatcgatgcggtgggttttttaaa(T)10ca(T)10attctacgatatctagttattaattatttgtttaatattgttcatcgg(T)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTGATGCACAGGGTAGAT | CGCAATGGGACTCTGGAAGT | Start: 623 |
| Tm | 59.534 | 60.036 | End: 826 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
|---|
| Sequence | ACTATCGATGCGGTGGGTTT | CTACCCTGTGCATCACGGTT | Start: 447 |
| Tm | 59.46 | 60.036 | End: 640 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
|---|
| Sequence | ACTATCGATGCGGTGGGTTT | TACCCTGTGCATCACGGTTC | Start: 447 |
| Tm | 59.46 | 60.037 | End: 639 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
|---|
| Sequence | CCGTGATGCACAGGGTAGAT | TCGCAATGGGACTCTGGAAG | Start: 623 |
| Tm | 59.534 | 59.75 | End: 827 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | ACTATCGATGCGGTGGGTTT | ATCTACCCTGTGCATCACGG | Start: 447 |
| Tm | 59.46 | 59.534 | End: 642 |
| Size | 20 | 20 | 196 |
(A)16caataatgattgtattaattttaaacttatattctcatttacttcccagtgtcatgaa(ATT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTGATGCACAGGGTAGAT | CGCAATGGGACTCTGGAAGT | Start: 623 |
| Tm | 59.534 | 60.036 | End: 826 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
|---|
| Sequence | ACTATCGATGCGGTGGGTTT | CTACCCTGTGCATCACGGTT | Start: 447 |
| Tm | 59.46 | 60.036 | End: 640 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
|---|
| Sequence | ACTATCGATGCGGTGGGTTT | TACCCTGTGCATCACGGTTC | Start: 447 |
| Tm | 59.46 | 60.037 | End: 639 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
|---|
| Sequence | CCGTGATGCACAGGGTAGAT | TCGCAATGGGACTCTGGAAG | Start: 623 |
| Tm | 59.534 | 59.75 | End: 827 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | ACTATCGATGCGGTGGGTTT | ATCTACCCTGTGCATCACGG | Start: 447 |
| Tm | 59.46 | 59.534 | End: 642 |
| Size | 20 | 20 | 196 |
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