ssr_primer
Primer Details for NODE_381922_length_714_cov_1.455538 |
|---|
| (TGT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGTGGGTCGTGGTCAATCT | AGCTGTCATGACGCCAATGA | Start: 27 |
| Tm | 59.965 | 60.036 | End: 194 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | GTGTGGGTCGTGGTCAATCT | TGCAACTACAGCTACAGGGC | Start: 27 |
| Tm | 59.965 | 60.036 | End: 128 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | TGACATTCTTGGACGCTCCC | AGCTGTCATGACGCCAATGA | Start: 60 |
| Tm | 60.036 | 60.036 | End: 194 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | GTGTGGGTCGTGGTCAATCT | ACAACAACACAGGCTGAGCT | Start: 27 |
| Tm | 59.965 | 60.107 | End: 268 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | GCCCTGTAGCTGTAGTTGCA | ACAACAACACAGGCTGAGCT | Start: 109 |
| Tm | 60.036 | 60.107 | End: 268 |
| Size | 20 | 20 | 160 |
(C)11atctccccctcgggatgca(C)21: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGTGGGTCGTGGTCAATCT | AGCTGTCATGACGCCAATGA | Start: 27 |
| Tm | 59.965 | 60.036 | End: 194 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | GTGTGGGTCGTGGTCAATCT | TGCAACTACAGCTACAGGGC | Start: 27 |
| Tm | 59.965 | 60.036 | End: 128 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | TGACATTCTTGGACGCTCCC | AGCTGTCATGACGCCAATGA | Start: 60 |
| Tm | 60.036 | 60.036 | End: 194 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | GTGTGGGTCGTGGTCAATCT | ACAACAACACAGGCTGAGCT | Start: 27 |
| Tm | 59.965 | 60.107 | End: 268 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | GCCCTGTAGCTGTAGTTGCA | ACAACAACACAGGCTGAGCT | Start: 109 |
| Tm | 60.036 | 60.107 | End: 268 |
| Size | 20 | 20 | 160 |
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